25-OH vitamin D nmol/L → ng/mL

Convert 25-hydroxyvitamin D from nmol/L to ng/mL

Sources last checked: 2026-10-13

25-OH vitamin D nmol/L → ng/mL

Convert 25-hydroxyvitamin D from nmol/L to ng/mL

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Applies to 25-hydroxyvitamin D (the status marker), not to 1,25-dihydroxyvitamin D, which is reported in pmol/L or pg/mL.

Source: AMA Manual of Style, 11th ed. (Oxford Academic) — Units of measure converter, Table 2: Selected laboratory tests and conversion factors.Open

What is the 25-OH vitamin D nmol/L → ng/mL?

25-hydroxyvitamin D, the circulating marker of vitamin D status, is reported in nmol/L in most European labs and in ng/mL in Germany (often), Turkey and the USA. The AMA Manual of Style SI table gives 2.496 nmol/L per ng/mL, from the molar mass of 25-hydroxyvitamin D₃ (400.6 g/mol).

When to use it

  • Comparing vitamin D results with thresholds expressed in the other unit
  • Reading guidelines that use ng/mL (US) or nmol/L (Europe)
  • Explaining results to patients

When not to use it

  • 1,25-dihydroxyvitamin D (calcitriol), measured in pmol/L or pg/mL
  • Vitamin D supplement doses (IU or µg) — different quantity

How it is calculated

25-OH-D (ng/mL) = 25-OH-D (nmol/L) ÷ 2.496 (≈ ÷ 2.5)

Clinical pearls and limitations

  • Applies to 25-hydroxyvitamin D (the status marker), not to 1,25-dihydroxyvitamin D, which is reported in pmol/L or pg/mL.
  • Quick check: nmol/L ÷ 2.5 ≈ ng/mL (50 nmol/L ≈ 20 ng/mL; 75 ≈ 30).
  • Assays differ by up to 10–20 %; follow-up should use the same lab.
  • Supplement dose conversion: 1 µg = 40 IU.

Frequently asked questions

How do I convert vitamin D from nmol/L to ng/mL?

Divide by 2.496 (≈ 2.5). Example: 50 nmol/L ≈ 20 ng/mL.

What is 30 ng/mL vitamin D in nmol/L?

About 75 nmol/L.

Is the conversion the same for vitamin D2 and D3?

Labs report total 25-OH-D with the D₃-based factor; the small molar mass difference of D₂ is ignored in practice.

Evidence and guidelines

  1. Primary source: AMA Manual of Style, 11th ed. (Oxford Academic) — Units of measure converter, Table 2: Selected laboratory tests and conversion factors.
  2. PubChem compound summary: calcifediol (25-hydroxyvitamin D₃) (CID 5283731), molecular weight 400.6 g/mol.

Have a follow-up question?

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For healthcare professionals and medical students. These tools support, and do not replace, clinical judgement. Always check results against the original source and your local protocols.